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Nile Red

Alfa Chemistry offers Nile Red products for various research purposes. Please contact us by email if you do not find the specification you are looking for on this page.

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Catalog Number
ACM7385673
Product Name
Nile Red
https://resource.alfa-chemistry.com/structure/7385-67-3.gif
CAS
7385-67-3
Synonyms
9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
Molecular Weight
318.38
Molecular Formula
C20H18N2O2
Purity
>98.0%(HPLC)
Packing
500mg, 1g, 5g
Physical State
(20 deg.C) Solid
PubChem Substance ID
87573971
Appearance
Yellow to Brown to Dark green powder to crystal
MDL Number
MFCD00011639
Lambda
(max.) 550.0 to 554.0 nm(MeOH)
Absorbance
(E1%1cm) min. 1300(MeOH, 550.0 to 554.0 nm)
Absorption Wavelength
(max.) 552(MeOH) nm
StorageConditions
Store at room temperature and dry
Structure
https://resource.alfa-chemistry.com/structure/7385-67-3.gif

Nile red is a lipophilic dye. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile Red does not fluoresce; however, in lipid-rich environments it fluoresces strongly, emitting colors ranging from deep red to deep gold. The dye is highly solvochromic, with emission and excitation wavelengths that vary with solvent polarity, and no fluorescence at all in polar media.

Nile red has applications in cell biology, it can be used as a membrane dye, can be easily observed using epifluorescence microscopy, and its excitation and emission wavelengths are often shared with red fluorescent proteins. Nile red has also been used as part of a sensitive detection process for microplastics in bottled water. Furthermore, Nile Red is an excellent candidate for the fabrication of membranes for different sensors to detect environmental changes such as taste, gas, pH, etc.

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